Journal of Dali University ›› 2020, Vol. 5 ›› Issue (2): 56-60.DOI: 10. 3969 / j. issn. 2096-2266. 2020. 02. 012
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Shen Lei 1, 2 , Wu Zhuo 2 , Liu Xiaoyi 3 , Li Ying 2 , Li Qiujing 2
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Abstract: Objective: To study the influence of Xinmailong Injection(XML)on microglia activation and subtype differentiation after middle cerebral artery occlusion/reperfusion(MCAO/R)in rats. Methods: After establishment of MCAO/R models, the medicines were given after 1 h reperfusion, 3 times in total and 24 h interval each time. Then, the influences of XML on Iba-1+ cellular density in ischemic penumbra were observed by immunofluorescence method, the effects of XML on the levels of CD16 and Arginase-1 gene expression in ischemic penumbra were measured by Q-PCR. Results: Compared with MCAO/R model group, XML significantly decreased activated microglia density in ischemic penumbra after MCAO/R in rats, reduced the levels of CD16 gene expression, while could not affect Arginase-1 gene expression. Conclusion: XML reduced microglia activation in ischemic penumbra, decreased the transformation of microglia to M 1 subtype, but not affect microglia transformation to M 2 subtype.
Key words: Xinmailong Injection, cerebral ischemia/reperfusion, microglia, M 1 subtype, M 2 subtype
Shen Lei,Wu Zhuo,Liu Xiaoyi,Li Ying,Li Qiujing . Influence of Xinmailong Injection on Microglia Activation and Subtype Differentiation after Cerebral#br# Ischemia/Reperfusion in Rats[J]. Journal of Dali University, 2020, 5(2): 56-60.
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URL: http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/10. 3969 / j. issn. 2096-2266. 2020. 02. 012
http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/Y2020/V5/I2/56